Premium
Efficient enrichment of intact phosphorylated proteins by modified immobilized metal‐affinity chromatography
Author(s) -
Dubrovska Anna,
Souchelnytskyi Serhiy
Publication year - 2005
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200500002
Subject(s) - affinity chromatography , phosphorylation , chemistry , chromatography , proteomics , protein phosphorylation , biochemistry , enzyme , protein kinase a , gene
Phosphoproteome studies are hampered by the lack of methods which allow a comprehensive and fast analysis of intact phosphoproteins. Here we describe an immobilized metal‐affinity chromatography (IMAC)‐based technique for the enrichment of phosphorylated proteins, which allows recovery of up to 90% of phosphoproteins. This technique is compatible with 2‐DE and can be applied to cultured cells and tissues.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom