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Proteomic analysis of the Gallus gallus embryo at stage‐29 of development
Author(s) -
Agudo David,
GómezEsquer Francisco,
DíazGil Gema,
MartínezArribas Fernando,
Delcán José,
Schneider José,
Palomar María Angustias,
Linares Rafael
Publication year - 2005
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200402056
Subject(s) - embryo , proteome , biology , computational biology , proteomics , embryogenesis , identification (biology) , genetics , gene , botany
The chicken ( Gallus gallus ) is one of the primary models for embryological and developmental studies. In order to begin to understand the molecular mechanisms underlying the normal and abnormal development of the chicken, we used 2‐DE to construct a whole‐embryo proteome map. Proteins were separated by IEF on IPG strips, and by 11% SDS‐PAGE) gels. Protein identification was performed by means of PMF with MALDI‐TOF‐MS. In all, 105 protein spots were identified, 35 of them implicated in embryo development, 10 related with some diseases, and 16, finally, being proteins that have never been identified, purified or characterized in the chicken before. This map will be updated continuously and will serve as a reference database for investigators, studying changes at the protein level under different physiological conditions.