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Proteome of endothelial cell‐derived procoagulant microparticles
Author(s) -
Banfi Cristina,
Brioschi Maura,
Wait Robin,
Begum Shajna,
Gianazza Elisabetta,
Pirillo Angela,
Mussoni Luciana,
Tremoli Elena
Publication year - 2005
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200402017
Subject(s) - microbiology and biotechnology , population , proteome , chemistry , coagulation , proteomics , cell adhesion , biology , endothelial stem cell , cell , biochemistry , in vitro , psychology , demography , psychiatry , sociology , gene
Abstract Microparticles (MP) are small membrane vesicles that are released from cells upon activation or during apoptosis. Cellular MP in body fluids constitute a heterogeneous population, differing in cellular origin, numbers, size, antigenic composition and functional properties. MP support coagulation by exposure of tissue factor (TF), the initiator of coagulation in vivo . Moreover, MP may transfer bioactive molecules to other cells, thereby stimulating them to produce cytokines, cell‐adhesion molecules, growth factors and TF, and modulate endothelial functions. However, a comprehensive characterization of the antigenic composition of MP has been poorly defined. This study describes the protein composition of endothelial cell (EC)‐derived MP (EMP) using a proteomic approach. MS analysis indicated the presence of newly described protein such as metabolic enzymes, proteins involved in adhesion and fusion processes, members of protein folding event, cytoskeleton associated proteins and nucleosome. In conclusion, circulating EMP behave as an actual storage pool, able to disseminate blood‐borne TF activity and other bioactive effectors, as confirmed by our experiments showing an increased procoagulant activity of EC exposed to EMP.