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Self‐made frits for nanoscale columns in proteomics
Author(s) -
Maiolica Alessio,
Borsotti Dario,
Rappsilber Juri
Publication year - 2005
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200402010
Subject(s) - frit , materials science , nanoscopic scale , chromatography , nanotechnology , chemistry , composite material
We report here the production of self‐made frits for nano‐columns. The frits introduce a minor dead volume and can be placed in capillaries with a wide range of diameters (20–250 µm tested) in an extremely simple and low‐cost procedure. The obtained columns appear to be comparable to “no‐frit” columns with near‐ideal chromatographic characteristics. We expect that this frit will be useful for the spotting of gradients onto MALDI plates but also where special ESI set‐ups do not allow for “no‐frit” solutions.