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Proteome analysis of combined effects of androgen and estrogen on the mouse mammary gland
Author(s) -
Zhang Lijun,
Liu Xin,
Zhang Jian,
Cao Rui,
Lin Ying,
Xie Jinyun,
Chen Ping,
Sun Yi,
Li Daiqiang,
Liang Songping
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200402007
Subject(s) - dihydrotestosterone , estrogen , androgen , proteome , endocrinology , medicine , biology , hormone , mammary gland , marmoset , hyperplasia , breast cancer , bioinformatics , cancer , paleontology
Abstract We and other investigators have previously shown that estrogen and androgen have synergistic effects on the growth of mammary epithelial ducts and alveoli in the Noble mouse. However, the molecular mechanisms behind the synergy are unknown. In the present study, we treated female FVB mice with 17‐estrodial (E2) and 5‐dihydrotestosterone‐bezonate (DHT‐B) using slow‐releasing hormone pellets for 7 months. Dissection showed that hormone treatment caused atypical hyperplasia of mammary ducts and alveoli. A functional proteomic approach was used to study the holistic protein changes in mammary glands. 2‐DE was used to separate proteins. Twenty‐five protein spots that were differentially expressed in hormone‐treated tissues compared to the control were identified by MALDI‐TOF‐MS, and ESI‐quadrupole‐TOF‐MS, which include some proteins that are correlative with response to estrogen and androgen stimulation, cells differentiation and growth, signal transduction, metabolism, etc . Real‐time RT‐PCR was carried out to verify the different expression. These results offered some clues to understand the function of E2 and DHT‐B.