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Endocrine disrupting effect of di‐(2‐ethylhexyl)phthalate on female rats and proteome analyses of their pituitaries
Author(s) -
Hirosawa Narumi,
Yano Kazuyuki,
Suzuki Yuko,
Sakamoto Yasushi
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200401344
Subject(s) - phthalate , estrous cycle , medicine , luteinizing hormone , endocrinology , endocrine system , hormone , proteome , follicle stimulating hormone , chemistry , biology , biochemistry , organic chemistry
Di‐(2‐ethylhexyl)phthalate (DEHP) is a plasticizer and a ubiquitous environmental contaminant that may have adverse effects on human reproductive health. We examined the long‐term exposure effects of DEHP on female rats and observed a strong effect on estrous cyclicity that produced a continuous diestrous stage. We found that the serum estradiol, follicle‐stimulating hormone (FSH), pituitary FSH and luteinizing hormone levels were significantly reduced in the treated rats. To examine on the endocrine disrupting effects, we performed proteome‐based analyses of their pituitaries, and found two proteins with remarkably reduced their levels. They were identified as the valosin‐containing peptide/p97 (VCP/p97) and UMP‐CMP kinase and their average protein spot intensities on statistical analysis of the spots differences of the treated/control rats were 0.13 and 0.21, respectively. Furthermore, there were 14 other proteins that had significantly changed levels, and their average protein spot intensities were in a range of 0.26 to 0.50 in 13 proteins and 2.74 in one. The reduction of in level of 7 proteins seems to be related to the intracellular protein transporting pathway, and it appears to suggest a slow down of gonadotrophin‐releasing capability. Reduction of gonadotrophin release in the pituitary seems to lead to a decrease of serum estradiol level and continuous diestrous stage in estrous cyclicity.