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Proteomics‐based analysis of a counter‐oxidative stress system in Porphyromonas gingivalis
Author(s) -
Okano Soichiro,
Shibata Yasuko,
Shiroza Teruaki,
Abiko Yoshimitsu
Publication year - 2006
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200401338
Subject(s) - porphyromonas gingivalis , hemin , oxidative stress , microbiology and biotechnology , groel , chronic periodontitis , bacteroidaceae , oxidative phosphorylation , anaerobic exercise , proteomics , superoxide dismutase , chemistry , proteome , biology , periodontitis , bacteria , biochemistry , gene , heme , medicine , escherichia coli , enzyme , genetics , physiology
Porphyromonas gingivalis is a Gram‐negative anaerobic pathogen associated with chronic periodontitis. Although anaerobic, P. gingivalis exhibits a high degree of aerotolerance, which enables it to survive within periodontal pockets. The aim of the present study was to examine the effect of oxidative stress on protein expression in P. gingivalis to obtain a better understanding of the mechanism underlying its aerotolerance. To accomplish this, P. gingivalis cells were grown under conditions of hemin limitation (0.01 μg/mL) to avoid the oxygen protective effect of hemin on oxidative stress. The proteins were then extracted from cultures either left untreated or subjected to oxidative stress and separated by 2‐DE. The resultant protein expression profiles were examined by image scanning, and those found to differ depending on the presence or absence of aeration were subjected to MALDI‐MS and then analyzed using the ORF database of P. gingivalis W83 from The Institute of Genomic Research. Oxidative stress was found to affect the expression of numerous proteins in P. gingivalis cells. In particular, the levels of HtpG, GroEL, DnaK, AhpC, TPR domain protein, and trigger factor were substantially increased.