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Immunoproteomics of outer membrane proteins and extracellular proteins of Shigella flexneri 2a 2457T
Author(s) -
Ying Tianyi,
Wang Hengliang,
Li Mingzhu,
Wang Junjun,
Wang Jie,
Shi Zhaoxing,
Feng Erling,
Liu Xiankai,
Su Guofu,
Wei Kaihua,
Zhang Xuemin,
Huang Peitang,
Huang Liuyu
Publication year - 2005
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200401326
Subject(s) - shigella flexneri , proteome , bacterial outer membrane , proteomics , biology , extracellular , microbiology and biotechnology , antigen , membrane protein , biochemistry , immunology , escherichia coli , membrane , gene
Shigella flexneri 2a is an important pathogen causing bacillary dysentery in humans. In order to investigate any potential vaccine candidate proteins present in outer membrane proteins (OMPs) and extracellular proteins of S. flexneri 2a 2457T, we use the proteome mapping and database analyzing techniques. A subproteome map and database of OMPs were established first. One hundred and nine of the total 126 marked spots were cut out and processed to MALDI‐TOF‐MS and PMF. Eighty‐seven spots were identified and they represented 55 OMP entries. Furthermore, immunoproteomics analysis of OMPs and extracellular proteins were performed. Total of 34 immunoreactive spots were identified, in which 22 and 12 were from OMPs and extracellular proteins, respectively. Eight novel antigens were found and some of these antigens may be potential vaccine candidate proteins. These results are useful for future studying of pathogenicity, vaccine, and novel antibacterial drugs. Maps and tables of all identified proteins are available on the Internet at www.proteomics.com.cn .