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2‐D reference map of Bacillus anthracis vaccine strain A16R proteins
Author(s) -
Wang Junjun,
Ying Tianyi,
Wang Hengliang,
Shi Zhaoxing,
Li Mingzhu,
He Kun,
Feng Erling,
Wang Jie,
Yuan Jing,
Li Ting,
Wei Kaihua,
Su Guofu,
Zhu Houchu,
Zhang Xuemin,
Huang Peitang,
Huang Liuyu
Publication year - 2005
Publication title -
proteomics
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200401322
Subject(s) - bacillus anthracis , bacillus cereus , bacillus thuringiensis , strain (injury) , biology , proteomics , computational biology , microbiology and biotechnology , gene , genetics , bacteria , anatomy
Bacillus anthracis has always been an important pathogen because it can cause lethal inhalational anthrax, and may be used as a bioweapon or by bioterrorists. In this study, a 2-DE reference map and database of B. anthracis A16R was constructed. In total, 534 spots were processed, and 406 spots representing 299 proteins were identified. Gel-estimated pIs and molecular masses mostly matched well with their theoretical predictions, but some discrepancies also existed. Spot and protein corresponding analysis revealed that post-translational modifications might be common in B. anthracis. Through the MASCOT search, the similarity of B. anthracis, B. cereus and B. thuringiensis was further verified by protein level and a possible annotation error in B. anthracis strain Ames 0581 genome was found. Proteins of energy metabolism, fatty acid and phospholipid metabolism, protein synthesis, and cellular processes represented a large part of the most abundant proteins. At the same time, 27 hypothetical proteins were experimentally proved. There were 28 proteins also identified as spore composition in recently spore-related research, which indicated that they might play some roles in different phases such as growth, sporulation and outgrowth. Maps and information about all identified proteins are available on the Internet at http://www.mpiib-berlin.mpg.de/2D-PAGE and http://www.proteomics.com.cn.