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Proteomic changes in the pressure overloaded right ventricle after 6 weeks in young rats: Correlations with the degree of hypertrophy
Author(s) -
Faber Matthijs J.,
Dalinghaus Michiel,
Lankhuizen Inge M.,
Bezstarosti Karel,
Dekkers Dick H. W.,
Duncker Dirk J.,
Helbing Willem A.,
Lamers Jos M. J.
Publication year - 2005
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200401313
Subject(s) - muscle hypertrophy , myofilament , desmin , ventricle , medicine , biology , pulmonary artery banding , endocrinology , myocyte , immunohistochemistry , vimentin
Right ventricular (RV) hypertrophy is an important problem in congenital heart disease. We determined the alterations in phenotype that occur in the initial phase of RV hypertrophy and their possible correlations with the degree of hypertrophy. Therefore, we performed a differential proteomic profiling study on RV hypertrophy using an animal model of pulmonary artery banding (PAB) in parallel with hemodynamic characterization. The RV homogenates were subfractionated in myofilament and cytoplasmic proteins, which subsequently were separated by two‐dimensional gel electrophoresis (2‐DE), excised, and analyzed by mass spectrometry (MS). The cytoplasmic fraction showed expression changes in metabolic proteins, indicative of a shift from fatty acid to glucose as a substrate for energy supply. Up‐regulation of three HSP‐27s (1.9‐, 1.7‐, and 3.5‐fold) indicated an altered stress response in RV hypertrophy. Detailed analysis by immunoblotting and MS showed that two of these HSP‐27s were at least phosphorylated on Ser15. The myofilament fraction showed up‐regulation of desmin and α‐B‐crystallin (1.4‐and 1.3‐fold, respectively). This alteration in desmin was confirmed by 1‐DE immunoblots. Certain differentially expressed proteins, such as HSP‐27, showed a significant correlation with the RV weight to the body weight ratio in the PAB rats, suggesting an association with the degree of hypertrophy.