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Improvements in two‐dimensional gel electrophoresis by utilizing a low cost “in‐house” neutral pH sodium dodecyl sulfate‐polyacrylamide gel electrophoresis system
Author(s) -
Graham David R. M.,
Garnham Christopher P.,
Fu Qin,
Robbins Jeffrey,
Van Eyk Jennifer E.
Publication year - 2005
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200401249
Subject(s) - tris , chromatography , sodium dodecyl sulfate , chemistry , electrophoresis , polyacrylamide gel electrophoresis , gel electrophoresis , hydroxymethyl , sodium , capillary electrophoresis , gel electrophoresis of proteins , polyacrylamide , two dimensional gel electrophoresis , biochemistry , proteomics , polymer chemistry , organic chemistry , enzyme , gene
Two‐dimensional gel electrophoresis (2‐DE) is widely used for initial protein separation in proteomics. Commercial products using neutral pH sodium dodecyl sulfate‐polyacrylamide gel electrophoresis ((SDS‐PAGE)/(Bis (2‐hydroxyethyl) imino‐tris (hydroxymethyl) methane‐HCl, or Bis‐Tris)) have greatly improved this technique, but cost and limited sizes restrict their applications. An “in‐house” system is presented, resulting in better resolution, separation, and new spot visualization and improved resolution when compared to Tris‐HCl gels. Their utility is demonstrated using albumin‐depleted serum samples, rabbit heart left ventricle, and human immunodeficiency virus type 1 (HIV‐1).