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The development of an algorithm for the mass spectral interpretation of phosphoproteins
Author(s) -
Zhao Yupeng,
Lin YenHan
Publication year - 2005
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200401162
Subject(s) - phosphoprotein , phosphorylation , peptide , algorithm , interpretation (philosophy) , ion , mass spectrometry , mass spectrum , computer science , chemistry , computational biology , combinatorial chemistry , biological system , biology , biochemistry , chromatography , organic chemistry , programming language
Extended from the peptide mapping method (Fenyo, D., Curr. Opin. Biotechnol. 2000, 11, 391–395) the proposed algorithm takes the mass information of a precursor ion to reconstruct all possible phosphorylated peptide sequences. The mass spectra of product ions from the corresponding precursor ion is then used and compared to the reconstructed sequences to deduce the most probable phosphoprotein. The proposed algorithm also predicts all possible combinations of phosphopeptides, which may serve as a clue for designing proper phosphorylation experiments to validate the existence of these peptides and the corresponding proteins.