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Towards royal jelly proteome
Author(s) -
Scarselli Roberta,
Donadio Elena,
Giuffrida Maria Gabriella,
Fortunato Donatella,
Conti Amedeo,
Balestreri Ettore,
Felicioli Romano,
Pinzauti Mauro,
Sabatini Anna Gloria,
Felicioli Antonio
Publication year - 2005
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200401149
Subject(s) - royal jelly , proteome , honey bee , biology , serine protease , protease , proteomics , pollen , bee pollen , honey bees , gel electrophoresis , two dimensional gel electrophoresis , botany , enzyme , biochemistry , gene
The recent availability of the honey‐bee Apis mellifera genome and trascriptome of both the female castes, has stimulated new efforts in investigating the protein composition of royal jelly (RJ), its role in caste differentiation and its quality and typicality by a proteomic approach. This study is aimed both to separate and identify proteins of royal jelly and to detect some of them in honey‐bee pollen‐bread by using two‐dimensional gel electrophoresis, mass spectrometry and by de novo sequencing. All the identified proteins belonged to the Apis mellifera genome. Apalbumin 1 was also confirmed to be present in honey‐bee pollen‐bread where the presence of apalbumin 2 was also found. In addition several fragments of apalbumin 1 and apalbumin 3 were also found in RJ. These could be the result of protease activity other than that of serine‐protease. This study is a contribution to the description of royal jelly proteome.