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Proteomic identification of Ku70/Ku80 autoantigen recognized by monoclonal antibody against hepatocellular carcinoma
Author(s) -
Luk John M.,
Su Yvonne C. F.,
Lam Stanley C. T.,
Lee Carol K.,
Hu Mei Yu,
He Qing Yu,
Lau George K.,
Wong Fred W. S.,
Fan SheungTat
Publication year - 2005
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200401084
Subject(s) - monoclonal antibody , hepatocellular carcinoma , identification (biology) , antibody , cancer research , computational biology , biology , immunology , medicine , botany
A murine monoclonal antibody (mAb), CLD3 (IgG 1 ,κ), was generated against hepatocellular carcinoma (HCC). Both immunofluorescence and immunohistochemical assays indicated the reactivity of CLD3 mAb localized at the nucleus and/or cytoplasm of tumorigenic HCC cell lines as well as in liver cancer tissues. By immunoprecipitation and using the matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry approach, the antigenic specificity of CLD3 was determined to be heterodimeric Ku70 and Ku80 autoantigen, which was confirmed by Western blotting.