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The first two‐dimensional reference map of the fission yeast, Schizosaccharomyces pombe proteins
Author(s) -
Sun Namkyu,
Jang Jaewon,
Lee Seunghee,
Kim Sunkyung,
Lee Seunghyun,
Hoe KwangLae,
Chung KyungSook,
Kim DongUk,
Yoo HyangSook,
Won Misun,
Song Kyung Bin
Publication year - 2005
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200401053
Subject(s) - schizosaccharomyces pombe , mass spectrometry , silver stain , chemistry , isoelectric focusing , spots , proteome , tandem mass spectrometry , proteomics , gel electrophoresis , time of flight mass spectrometry , yeast , biochemistry , schizosaccharomyces , chromatography , biology , microbiology and biotechnology , saccharomyces cerevisiae , ionization , enzyme , ion , organic chemistry , gene
Cytosolic proteins of Schizosaccharomyces pombe were separated by two‐dimensional (2‐D) gel electrophoresis, to construct the first 2‐D reference map. In the p I range 4–7, more than 500 spots were detected by silver staining, and 70 different proteins corresponding to 111 spots were identified by matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry and tandem mass spectrometry, where necessary. In the p I range 6–9, approximately 330 spots were detected, and 31 proteins corresponding to 38 spots were identified by mass spectrometry. More than 50% of the identified proteins were involved in amino acid, carbohydrate or nucleotide metabolism, and energy production. A second large group of identified proteins comprises heat shock and other stress related proteins and chaperones.