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Proteomic analysis of livers of patients with primary hepatolithiasis
Author(s) -
Nabetani Takuji,
Tabuse Yo,
Tsugita Akira,
Shoda Junichi
Publication year - 2005
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200401039
Subject(s) - hepatolithiasis , difference gel electrophoresis , chaperonin , proteome , biology , proteomics , gel electrophoresis , bile duct , microbiology and biotechnology , two dimensional gel electrophoresis , silver stain , pathology , biochemistry , hepatectomy , medicine , gene , surgery , resection
Abstract Primary hepatolithiasis or intrahepatic calculi (IHC), which is characterized by the formation of gallstones in the intrahepatic bile duct, is an intractable liver disease and suspected to be one of the causes of cholangiocellular carcinoma. To obtain an insight into the disease, we performed proteomic analysis of liver tissue specimens of paired affected and unaffected hepatic segments from patients with primary hepatolithiasis by two‐dimensional gel electrophoresis followed by identification of proteins. For the specimens from the unaffected segments, 125 spots out of 613 spots were identified, defining 83 unique protein names. For the specimens from the affected segments, 102 spots out of 671 spots were identified, defining 74 unique protein names. To further precisely compare, we used two‐dimensional fluorescence difference gel electrophoresis. Consequently we identified 12 up‐regulated proteins and 21 down‐regulated proteins. The up‐regulated proteins contained the proteins related to liver fibrosis and to cellular oxidoreduction. The down‐regulated proteins contained RAF kinase inhibitor protein, chaperonin and proteins related to principal liver function.