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Identification of target proteins of N ‐acetylglucosaminyl‐transferase V and fucosyltransferase 8 in human gastric tissues by glycomic approach
Author(s) -
Kim YongSam,
Hwang Soo Young,
Oh Sejeong,
Sohn Hosung,
Kang HyeYeon,
Lee JeongHwa,
Cho EunWie,
Kim JinYoung,
Yoo Jong Shin,
Kim NamSoon,
Kim CheorlHo,
Miyoshi Eiji,
Taniguchi Naoyuki,
Ko Jeong Heon
Publication year - 2004
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200401026
Subject(s) - western blot , biochemistry , proteomics , carcinogenesis , blot , cancer , proteolysis , biology , gel electrophoresis , fucosyltransferase , chemistry , microbiology and biotechnology , enzyme , genetics , gene
To understand better the mechanism underlying gastric cancer and search for potential markers for gastric cancer prognosis, the proteomic analysis of gastric cancer tissues was conducted using two‐dimensional gel electrophoresis and lectin blot, followed by electrospray ionization‐mass spectrometry. These approaches permitted identification of glyco‐ or putative glycosylated proteins which may be associated with tumorigenesis. The proteins identified include molecules involved in sugar metabolism, signal transduction, proteolysis, and stress, as well as several unknown proteins, which were aberrantly glycosylated as evidenced by the L ‐phytohemagglutinin blot.