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Proteomic analysis revealed a strong association of a high level of α1‐antitrypsin in gastric juice with gastric cancer
Author(s) -
Lee Kyunghee,
Kye Minsu,
Jang Joung Soon,
Lee Ok Jae,
Kim Taehyo,
Lim Dongbin
Publication year - 2004
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200400960
Subject(s) - pepsin , zymography , cancer , intestinal metaplasia , gastroenterology , protease , atrophic gastritis , medicine , pathology , biology , gastritis , enzyme , stomach , biochemistry , matrix metalloproteinase
To investigate the pathology of gastric disorders, we compared the proteomic patterns of gastric juice from patients with various gastric disorders. In healthy subjects pepsin A, pepsin B and gastric lipase were the major proteins detected by two‐dimensional gel electrophoresis. These digestive enzymes were not detected in 60% of gastric cancer cases (18 out of 30 analyzed cases). Interestingly, an extraordinary amount of α 1 ‐antitrypsin was observed in these cases. In contrast to gastric cancer cases, α 1 ‐antitrypsin was detected in only 5% of patients (three out of 56) with chronic atrophic gastritis, and the detection frequency went up as the disease developed (one of four intestinal metaplasia cases, two of seven tubular adenoma cases, a single examined case of hyperplastic polyp and 60% of gastric cancer). Zymography showed that a 60 kDa protease strongly associated with α 1 ‐antitrypsin and mass spectrometric analysis revealed that the gastric α 1 ‐antitrypsin was a protease‐cleaved form. Our data suggest that α 1 ‐antitrypsin and 60 kDa protease may serve as good diagnostic and prognostic markers for conditions associated with gastric cancer.