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Proteomic analysis of the proliferative and secretory phases of the human endometrium: Protein identification and differential protein expression
Author(s) -
DeSouza Leroi,
Diehl Georg,
Yang Eric C.C.,
Guo Jingzhong,
Rodrigues Mary Joe,
Romaschin Alexander D.,
Colgan Terence J.,
Siu K.W. Michael
Publication year - 2005
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200400920
Subject(s) - secretory protein , endometrium , biology , proteomics , proteome , gene expression , protein subunit , gene , microbiology and biotechnology , chemistry , biochemistry , endocrinology
Proteomic analyses of the proliferative and secretory phases of the human endometrium were carried out to identify proteins and discover differentially expressed proteins using isotope‐coded affinity tags, three stages of chromatographic separation and online tandem mass spectrometry (MS/MS). From an initial list of 346 proteins identified by ProICAT, manual inspection of MS/MS spectra and confirmatory searches pared the list down to 119 positively identified proteins. Only five of the proteins showed consistent differential expression. The utility of some of these proteins as indicators of true differential expression in the endometrium is open to discussion. The two proteins with unquestionable differential expressions in the secretory endometrium are: glutamate NMDA receptor subunit zeta 1 precursor and FRAT1. Some of the proteins that show no differential expression have previously been examined in gene‐expression studies with similar conclusions.