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Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two‐dimensional electrophoresis
Author(s) -
Luche Sylvie,
Santoni Véronique,
Rabilloud Thierry
Publication year - 2003
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200390037
Subject(s) - chemistry , chromatography , membrane protein , membrane , electrophoresis , biochemistry , solubilization , ether , gel electrophoresis , proteomics , organic chemistry , gene
The solubilizing power of various nonionic and zwitterionic detergents as membrane protein solubilizers for two‐dimensional electrophoresis was investigated. Human red blood cell ghosts and Arabidopsis thaliana leaf membrane proteins were used as model systems. Efficient detergents could be found in each class, i.e. with oligooxyethylene, sugar or sulfobetaine polar heads. Among the commercially available nonionic detergents, dodecyl maltoside and decaethylene glycol mono hexadecyl ether proved most efficient. They complement the more classical sulfobetaine detergents to widen the scope of useful detergents for the solubilization of membrane proteins in proteomics.