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Protein identification in cerebrospinal fluid using packed capillary liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry
Author(s) -
Ramström Margareta,
Palmblad Magnus,
Markides Karin E.,
Håkansson Per,
Bergquist Jonas
Publication year - 2003
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200390027
Subject(s) - fourier transform ion cyclotron resonance , mass spectrometry , chromatography , chemistry , top down proteomics , ion cyclotron resonance , cerebrospinal fluid , analytical chemistry (journal) , selected ion monitoring , protein mass spectrometry , fourier transform , nuclear magnetic resonance , tandem mass spectrometry , ion , cyclotron , gas chromatography–mass spectrometry , physics , medicine , pathology , organic chemistry , quantum mechanics
The identification and characterization of proteins in complex biological samples such as body fluids, require powerful and reliable tools. Mass spectrometry is today one of the most important methods in such research. This paper reports on the results from the first experiment where a tryptic digest of cerebrospinal fluid was analyzed applying reversed phase liquid chromatography coupled on‐line to a 9.4 T Fourier transform ion cyclotron resonance mass spectrometer. In total, 70 204 peaks were detected, which originated from 16 296 isotopic clusters corresponding to 6551 unique peptide masses. From these masses, 39 proteins were identified in the sample. The amount of sample required for one experiment corresponds to 32 μL of cerebrospinal fluid.