Premium
Progress in the definition of a reference human mitochondrial proteome
Author(s) -
Lescuyer Pierre,
Strub JeanMarc,
Luche Sylvie,
Diemer Hélène,
Martinez Pascal,
Van Dorsselaer Alain,
Lunardi Joël,
Rabilloud Thierry
Publication year - 2003
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200390024
Subject(s) - proteome , computational biology , biology , organelle , compartmentalization (fire protection) , proteomics , human proteome project , mitochondrion , bioinformatics , microbiology and biotechnology , biochemistry , gene , enzyme
Owing to the complexity of higher eukaryotic cells, a complete proteome is likely to be very difficult to achieve. However, advantage can be taken of the cell compartmentalization to build organelle proteomes, which can moreover be viewed as specialized tools to study specifically the biology and “physiology” of the target organelle. Within this frame, we report here the construction of the human mitochondrial proteome, using placenta as the source tissue. Protein identification was carried out mainly by peptide mass fingerprinting. The optimization steps in two‐dimensional electrophoresis needed for proteome research are discussed. However, the relative paucity of data concerning mitochondrial proteins is still the major limiting factor in building the corresponding proteome, which should be a useful tool for researchers working on human mitochondria and their deficiencies.