Premium
Proteomic analysis of Deinococcus radiodurans recovering from γ‐irradiation
Author(s) -
Zhang Chunchao,
Wei Jianfeng,
Zheng Zhiguo,
Ying Nanjiao,
Sheng Duohong,
Hua Yuejin
Publication year - 2005
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300875
Subject(s) - deinococcus radiodurans , computational biology , chemistry , proteomics , irradiation , biology , microbiology and biotechnology , biochemistry , dna , gene , physics , nuclear physics
Abstract In order to reveal the mechanisms of the extreme radioresistance and DNA repair in Deinococcus radiodurans , we examined proteome changes in a wild‐type strain following γ‐irradiation using two‐dimensional polyacrylamide gel electrophoresis and Silver‐staining. The expression levels of 26 protein spots showed significant changes under radiation stress. Of these spots, 21 were identified with peptide mass fingerprinting using matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry after tryptic in‐gel digestion. These proteins exhibited various cellular functions, including (i) translation; (ii) transcription; (iii) signal transduction; (iv) post‐translational modification, protein turnover, chaperones; (v) carbohydrate transport and metabolism; (vi) energy production and conversion; (vii) nucleotide transport and metabolism; (viii) inorganic ion transport and metabolism; (ix) DNA replication, recombination and repair; and (x) yet unknown. Most of the proteins have not previously been reported to be relevant to radioresistance.