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Proteomic approach to identify acute phase response‐related proteins with low molecular weight in loach skin following injury
Author(s) -
Peng Xuanxian,
Wu Yajun,
Chen Jingan,
Wang Sanying
Publication year - 2004
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300864
Subject(s) - proteome , ribosomal protein , microbiology and biotechnology , proteomics , blot , gel electrophoresis , chemistry , biochemistry , biology , rna , ribosome , gene
Proteome analysis by two‐dimensional gel electrophoresis (2‐DE) together with mass spectrometry was applied to screen acute phase response (APR)‐related proteins with low molecular weight in loach skin following injury. Furthermore, Western blotting and function tests were applied to confirm the results obtained from the proteomic study. Fifteen APR‐related proteins with sixteen spots (PLA with two spots) on a 2‐DE map were identified in this study. Furthermore, six were known acute phase proteins including galactose‐binding lectin (GBL), lysozyme, C3, CD59, double PLA and 50s ribosomal protein; while ATP kinase, zinc finger protein 183, alpha‐neurotoxin homology, angiostatin, serine/threonine kinase, metalloproteinase inhibitor, regulator of G‐protein 4, cryptdin‐9 and disintegrin trigranin were found by our lab to be APR‐related proteins. In addition, our results suggest that proteomes with low molecular weight can be characterized by 2‐DE with a Tris‐tricine system followed by mass spectrometry.