Premium
Proteomic analysis of differential protein expression in neuropathic pain and electroacupuncture treatment models
Author(s) -
Sung Ho Joong,
Kim Yoon Suk,
Kim In Sik,
Jang SungWuk,
Kim Yoon Rim,
Na Doe Sun,
Han Ki Hoon,
Hwang Byung Gil,
Park Dong Suk,
Ko Jesang
Publication year - 2004
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300821
Subject(s) - electroacupuncture , neuropathic pain , proteomics , protein expression , medicine , neuroscience , physical medicine and rehabilitation , bioinformatics , computational biology , biology , pharmacology , acupuncture , pathology , biochemistry , alternative medicine , gene
Acupuncture has long been used for pain relief. Although recent studies have shown that acupuncture can reduce neuropathic pain, the mechanism of this effect is not clear and little information is available regarding proteins that are involved in the development of neuropathic pain and the effects of acupuncture. We have developed an animal model for neuropathic pain using young adult male Sprague‐Dawley rats. The model was confirmed by behavioral tests. Electroacupuncture (EA) treatment was applied to Zusanli (ST36) of neuropathic pain model to examine the analgesic effect of EA. The protein expression profile of the hypothalamus in both neuropathic pain and EA treatment models was analyzed using two‐dimensional electrophoresis‐based proteomics. We detected thirty‐six proteins that were differentially expressed in the neuropathic pain model compared with normal rats and that restored to normal expression levels after EA treatment. Twenty‐one of these proteins were identified in the MS‐FiT database and are involved in a number of biological processes, including inflammation, enzyme metabolism and signal transduction. Potential applications of our results include the identification and characterization of signaling pathways involved in EA treatment and further exploration of the role of selected identified proteins in the animal model.