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Two‐dimensional gel electrophoresis database of murine R1 embryonic stem cells
Author(s) -
Elliott Steven T.,
Crider David G.,
Garham Christopher P.,
Boheler Kenneth R.,
Van Eyk Jennifer E.
Publication year - 2004
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300820
Subject(s) - embryonic stem cell , biology , proteomics , induced pluripotent stem cell , cellular differentiation , gel electrophoresis , microbiology and biotechnology , cell culture , stem cell , population , genetics , gene , demography , sociology
Embryonic stem (ES) cell lines represent a population of undifferentiated pluripotent cells capable of multilineage differentiation in vitro. Although very useful for studying developmental processes, human ES cell lines have also been suggested as a potential and unlimited source for cellular transplantation and the treatment of human disease. The proteomic basis of embryonic stemness (pluripotentiality and multilineage differentiation) and the transitions that lead to specific cell lineages however, remain to be defined. As an important first step in defining these processes, we have performed a proteomic analysis of undifferentiated mouse R1 ES cell lines using pH 3–10, 4–7 and 6–11 two‐dimensional electrophoresis gels, matrix‐assisted laser desorption/ionization and tandem mass spectrometry. Of the 700 gel spots analyzed, 241 distinct protein species were identified corresponding to 218 unique proteins, with a significant proportion functionally related to protein expression.