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A method for the rapid depletion of albumin and immunoglobulin from human plasma
Author(s) -
Greenough Carrie,
Jenkins Rosalind E.,
Kitteringham Neil R.,
Pirmohamed Munir,
Park B. Kevin,
Pennington Stephen R.
Publication year - 2004
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300815
Subject(s) - albumin , antibody , human plasma , human albumin , immunoglobulin g , chromatography , immunoglobulin m , chemistry , blood proteins , immunology , medicine , biochemistry
In studies of the plasma proteome, the high abundance of proteins such as albumin and immunoglobulin impedes the investigation of lower abundance proteins that may be more suitable as biomarkers of disease. We report the specific removal of 98% of albumin and 80% of immunoglobulin heavy chain from human plasma by affinity chromatography, and the subsequent improvement in the number of spots detected and their resolution following two‐dimensional gel electrophoresis.