Premium
Use of surface‐enhanced laser desorption/ionization ‐time of flight to explore bacterial proteomes
Author(s) -
Barzaghi Dagania,
Isbister Jenefir D.,
Lauer Kimberly P.,
Born Timothy L.
Publication year - 2004
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300782
Subject(s) - proteome , surface enhanced laser desorption/ionization , proteomics , bacteria , chromatography , mass spectrometry , microbiology and biotechnology , chemistry , biology , computational biology , materials science , biochemistry , tandem mass spectrometry , protein mass spectrometry , genetics , gene
Surface‐enhanced laser desorption/ionization (SELDI)‐time of flight is a recent technology that allows proteomic analysis with limited material requirements. This characteristic makes it a valuable technique for microbiologists handling problematic samples, such as low cell number cultures. We compared three simple procedures for protein extraction from bacteria for compatibility with the ProteinChip Array; we also determined the amount of protein required for each analysis. The protocol for the SELDI analysis was evaluated by generating protein expression profiles of a Streptococcus pneumoniae strain grown in different conditions and those of different strains of the same species. The protocol also was successfully applied to a wide range of Gram positive and negative bacteria. The results of this study suggest the appropriateness of this technology for microorganism protein profiling as complementary or alternative to two‐dimensional gel electrophoresis.