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Heterogeneous nuclear ribonucleoprotein A2/B1 identified as an autoantigen in autoimmune hepatitis by proteome analysis
Author(s) -
Huguet Stèphanie,
Labas Valèrie,
DuclosVallee JeanCharles,
Bruneel Arnaud,
Vinh Joëlle,
Samuel Didier,
Johanet Catherine,
Ballot Eric
Publication year - 2004
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300757
Subject(s) - anti nuclear antibody , ribonucleoprotein , extractable nuclear antigens , polyacrylamide gel electrophoresis , antigen , proteome , gel electrophoresis , microbiology and biotechnology , autoimmune hepatitis , antibody , biology , chemistry , hepatitis , immunology , biochemistry , autoantibody , rna , gene , enzyme
Our aim was to characterize antinuclear antibody (ANA) targets in type 1 autoimmune hepatitis (AIH1) using a proteomic tool. ANA‐positive sera from 29 patients with AIH1 and 14 negative controls were analyzed by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and immunoblotting. The principal antigens identified were a 30 kDa triplet band which was recognized by 79% of patient sera and 14% of control sera, and a 36 kDa antigen which was recognized by 52% and 14% of sera, respectively. The latter antigen was studied in more detail using two‐dimensional electrophoresis (2‐DE) and mass spectrometry (MS). Its identification corresponded to heterogeneous nuclear ribonucleoprotein A2/B1. This paper presents the first description of this protein as an antigen in AIH1.