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Detection of regional changes in protein levels in the in vivo canine model of acute heart failure following ischemia‐reperfusion injury: Functional proteomics studies
Author(s) -
Sawicki Grzegorz,
Jugdutt Bodh I.
Publication year - 2004
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300746
Subject(s) - in vivo , creatine kinase , western blot , protein subunit , proteomics , ischemia , atp synthase , chemistry , microbiology and biotechnology , biology , biochemistry , medicine , enzyme , gene
In this study, we demonstrate the use of proteomics to detect regional differences in protein levels between the reperfused ischemic zone (IZ) and the nonischemic zone (NIZ) of dog hearts which were subjected to in vivo ischemia‐reperfusion injury. Using the two‐dimensional gel electrophoresis (2‐DE) technique, we identified five proteins that were differentially expressed in the IZ versus NIZ: (1) the α subunit of ATP synthase isoform precursor was decreased 1.71‐fold; (2) creatine kinase M chain was decreased 1.72‐fold; (3) NAD+‐isocitrate dehydrogenase, α subunit was increased 8.34‐fold; (4) ATP synthase D chain, mitochondrial was increased 3.02‐fold; (5) ventricular myosin light chain‐1 was decreased 2.02‐fold. Additionally, we found that the level of actin was decreased 2.6‐times in the IZ compared to the NIZ on Western blot analysis but was unchanged on 2‐DE.