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Double chip protein arrays using recombinant single‐chain Fv antibody fragments
Author(s) -
Gilbert Ilka,
Schiffmann Susanne,
Rubenwolf Susanne,
Jensen Kristian,
Mai Thao,
Albrecht Christian,
Lankenau Andreas,
Beste Gerald,
Blank Kerstin,
Gaub Hermann E.,
ClausenSchaumann Hauke
Publication year - 2004
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300736
Subject(s) - monoclonal antibody , antibody , chip , recombinant dna , immunoglobulin light chain , immunoassay , false positive paradox , chemistry , computational biology , microbiology and biotechnology , computer science , biology , immunology , biochemistry , telecommunications , gene , machine learning
Protein arrays permit the parallel analysis of many different markers in a small sample volume. However, the problem of cross‐reactivity limits the degree of multiplexing in parallel sandwich immunoassays (using monoclonal antibodies (mAbs)), meaning antibodies must be prescreened in order to reduce false positives. In contrast, we use a second chip surface for the local application of detection antibodies, thereby efficiently eliminating antibody cross‐reactions. Here, we illustrate the potential advantages of using single‐chain Fv fragments rather than mAbs as capture and detection molecules with this double chip technology.