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A convenient method to extract matrix‐assisted laser desorption/ionization mass spectrometry spectra from phosphate‐containing peptide mixtures
Author(s) -
Huang ShengYu,
Hsu JueLiang,
Morrice Nicholas A.,
Wu ChinJen,
Chen ShuHui
Publication year - 2004
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300717
Subject(s) - mass spectrometry , chemistry , chromatography , peptide , matrix assisted laser desorption/ionization , matrix (chemical analysis) , phosphate , sample preparation in mass spectrometry , desorption , mass spectrum , analytical chemistry (journal) , sample preparation , maldi imaging , ionization , electrospray ionization , ion , adsorption , organic chemistry , biochemistry
Here we report that the addition of HCl or HNO 3 to the matrix at a limited concentration dramatically increases the signal‐to‐noise ratio of the matrix‐assisted laser desorption/ionization mass spectrometry spectrum of phosphate‐containing peptide mixtures such as those obtained from an immobilised metal affinity capture eluent or a phosphate‐containing tryptic digest. These improved spectra permitted both peptide identification and the determination of protein phosphorylation sites. In comparison to existing methods for removing salts, this method requires less sample manipulation and thus less sample loss is expected.