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Dispensable role for interferon‐γ in the burn‐induced acute phase response: A proteomic analysis
Author(s) -
Duan Xunbao,
Yarmush David M.,
Jayaraman Arul,
Yarmush Martin L.
Publication year - 2004
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300696
Subject(s) - acute phase protein , haptoglobin , knockout mouse , serum amyloid a , cytokine , burn injury , serum amyloid a protein , chemistry , microbiology and biotechnology , immunology , medicine , inflammation , biology , receptor , surgery
We examined the role of the pleiotropic cytokine interferon‐γ (IFN‐γ) in initiating the burn injury‐induced acute phase response (APR). Two‐dimensional (2‐D) electrophoresis was used to obtain serum protein profiles from wild‐type (WT) and IFN‐γ knockout mice following sham‐burn or 20% burn injury. Serum 2‐D images from both groups of burn‐injured mice were characterized by the upregulation of a similar panel of protein spots. These included the three major murine acute phase proteins haptoglobin, serum amyloid A, and serum amyloid P, that were identified by matrix‐assisted laser desorption/ionization‐time of flight (MALDI‐TOF)‐mass spectrometry. Furthermore, the changes in the levels of these protein spots were very similar between these two groups of mice, as determined by image analysis. Other features of burn‐induced APR such as a decrease in total serum protein concentration, an elevated circulation level of the cytokine interleukin‐6 (IL‐6), and activation of the IL‐6 signal transduction protein STAT3 were also evaluated and found to be similar between wild‐type and IFN‐γ knockout mice. These results suggest a dispensable role of IFN‐γ in the induction of the hepatic APR in mice following burn injury.