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Analysis of proteins expressed in rat plasma exposed to dioxin using 2‐dimensional gel electrophoresis
Author(s) -
Son WonKyu,
Lee DoYoun,
Lee SungHan,
Joo WonA.,
Kim ChanWha
Publication year - 2003
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300605
Subject(s) - chemistry , electrospray ionization , mass spectrometry , gel electrophoresis , blood proteins , chromatography , biochemistry
Dioxins are a class of polyhalogenated aromatic hydrocarbons that induces a wide spectrum of toxic responses in animals. In this study, two groups of Sprague‐Dawley rats were exposed to 2,3,7,8‐tetrachlorobenzo‐ p ‐dioxin (TCDD); one group received short‐term exposure at a single dose of 1, 10, 20 or 50 μg/kg body weight and the other received long‐term exposure to a daily low dose of 0.01, 0.1, 1 or 2.5 μg/kg body weight for one month. Two‐dimensional electrophoresis was utilized to resolve the protein profile of rat plasma exposed to TCDD at different doses. One novel and three volume‐increased spots were identified and characterized by matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry and electrospray‐ionization on quadropole‐TOF2 mass spectrometry. The novel protein was identified as plasma glutathione peroxidase precursor and the volume‐increased proteins were cytokeratin 8 polypeptide, Ig lambda‐1 chain C region and Ig lambda‐2 chain C region. These proteins may be used as biomarkers to diagnose dioxin exposure and may help in understanding the toxic effects of dioxins.