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Improved Ruthenium II tris (bathophenantroline disulfonate) staining and destaining protocol for a better signal‐to‐background ratio and improved baseline resolution
Author(s) -
Lamanda Andreas,
Zahn Alain,
Röder Daniel,
Langen Hanno
Publication year - 2004
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300587
Subject(s) - ruthenium , tris , staining , resolution (logic) , chemistry , chromatography , analytical chemistry (journal) , biology , biochemistry , computer science , genetics , artificial intelligence , catalysis
In proteomics the ability to visualize proteins from electropherograms is essential. Here a new protocol for staining and destaining gels treated with Ruthenium II tris (bathophenantroline disulfonate) is presented. The method is compared with the silver‐staining procedure of Swain and Ross, the Ruthenium II tris (bathophenantroline disulfonate) stain described by Rabilloud (Rabilloud T., Strub, S. M. Luche, S., Girardet, S. L. et al. , Proteomics 2001, 1 , 699–704) and the SYPRO Ruby gel stain. The method offers a better signal‐to‐background ratio with improved baseline resolution for both sodium dodecyl sulfate‐polyacrylamide gels and two‐dimensional gels.