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Identification of tumor‐associated proteins in oral tongue squamous cell carcinoma by proteomics
Author(s) -
He QingYu,
Chen Jia,
Kung HsiangFu,
Yuen Anthony PoWing,
Chiu JenFu
Publication year - 2004
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300550
Subject(s) - tongue , cancer , heat shock protein , carcinoma , proteomics , pathology , cancer research , proteome , biomarker , medicine , biology , bioinformatics , biochemistry , gene
Oral tongue carcinoma is an aggressive tumor that particularly affects chronic smokers, drinkers and betel squid chewers. Patients often present symptoms at a late stage, and there is a high recurrence rate after treatment. In this article, we report the first proteomic analysis of oral tongue carcinoma to globally search for tumor related proteins. Apart from helping us to understand the molecular pathogenesis of the carcinoma, these proteins may also have potential clinical applications as biomarkers, enabling the tumor to be identified at an early stage in high risk individuals, treatment response to be predicted, and residual or recurrent carcinoma to be detected sooner after treatment. The protein expression profiles of ten oral tongue squamous cell carcinomas and their matched normal mucosal resection margins were examined by two‐dimensional gel electrophoresis and matrix‐assisted laser desorption/ionization‐time of flight mass spectroscopy. A number of tumor‐associated proteins including heat shock protein (HSP)60, HSP27, α B‐crystalline, ATP synthase β, calgranulin B, myosin, tropomyosin and galectin 1 were consistently found to be significantly altered in their expression levels in tongue carcinoma tissues, compared with their paired normal mucosae. The expression profile portrays a global protein alteration that appears specific to oral tongue cancer. The potential of utilizing these tumor related proteins for screening cancer and monitoring recurrence warrants further investigation.