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Proteome profiling and its use in metabolic and cellular engineering
Author(s) -
Han MeeJung,
Lee Sang Yup
Publication year - 2003
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300508
Subject(s) - proteome , bioproducts , profiling (computer programming) , proteomics , computational biology , metabolic engineering , biology , biochemical engineering , computer science , microbiology and biotechnology , bioinformatics , biochemistry , engineering , enzyme , biofuel , gene , operating system
Proteome profiling of microorganisms makes it possible to generate valuable knowledge that can be used for the development of metabolic and cellular engineering strategies, which consequently are used to enhance the yield and productivity of native or foreign bioproducts and to modify cellular properties to improve mid‐stream and down‐stream processes. Advances in two‐dimensional gel electrophoresis technology combined with mass spectrometry allow the creation of global scale proteome contents which can be used to elucidate valuable information on the dynamics of the metabolic, signaling and regulatory networks apart from understanding the physiological changes. In this paper, we review the approaches of exploiting the proteome profiling results to the development of the strategies for the metabolic and cellular engineering of microorganisms.