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C ‐Terminal sequencing method for peptides and proteins by the reaction with a vapor of perfluoric acid in acetic anhydride
Author(s) -
Miyazaki Kenji,
Tsugita Akira
Publication year - 2004
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300507
Subject(s) - acetic anhydride , chemistry , mass spectrometry , acetylation , acetic acid , peptide , amino acid , chromatography , organic chemistry , biochemistry , catalysis , gene
A successive C ‐terminal amino acid truncation reaction of peptides and proteins with a vapor generated from a low‐concentrated perfluoric acid in acetic anhydride is presented. The reaction products were analyzed with matrix‐assisted laser desorption/ionization‐time of flight mass‐spectrometry giving molecular mass ions of the C ‐terminal truncated peptides or proteins from which the C ‐terminal sequence information can be deduced. Acetylation reaction preceded the truncation reaction in order to protect the amino groups and other reactive groups in peptides and proteins, and after the truncation reaction, hydration reaction was carried out to afford cleaner mass spectra.