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Lung proteome alterations in a mouse model for nonallergic asthma
Author(s) -
Houtman René,
Krijgsveld Jeroen,
Kool Mirjam,
Romijn Edwin P.,
Redegeld Frank A.,
Nijkamp Frans P.,
Heck Albert J. R.,
HumpherySmith Ian
Publication year - 2003
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300469
Subject(s) - proteome , asthma , lung , biology , immunology , proteomics , computational biology , medicine , bioinformatics , genetics , gene
Abstract A mouse model for nonatopic asthma was employed to study the alterations of the lung proteome to gain insight into the underlying molecular mechanisms of disease pathophysiology post‐challenge. Lung samples from asthmatic and control mice were used to generate 24 high quality two‐dimensional electrophoresis gels wherein 2115 proteins were examined for disease relevance. In total, 23 proteins were significantly up‐ or down‐regulated following hapten‐challenge of dinitro‐fluorobenzene‐hypersensitive mice. Twenty proteins were identified by mass spectrometry, of which 18 could be linked to asthma related symptoms, such as stress and inflammation, lung detoxification, plasma exudation and/or tissue remodeling. As such, proteomics was clearly vindicated as a means of studying this complex disease phenomenon. The proteins found in this study may not necessarily play a role in the immunological mechanisms and/or pathophysiology of asthma development. However, they may prove useful as surrogate biomarkers for quantitatively monitoring disease state progression or response to therapy. The mathematics of achieving statistical confidence from low numbers of gel replicates containing large numbers of independent variables stress the need for high numbers of replicates to better sample the population of proteins revealed by two‐dimensional gel electrophoresis.