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“Two‐in‐one” gel for spot matching after two‐dimensional electrophoresis
Author(s) -
Wang YuenYuen,
Cheung PikYuen,
Wong ManSau,
Lo Samuel ChunLap
Publication year - 2003
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300423
Subject(s) - silver stain , staining , gel electrophoresis , two dimensional gel electrophoresis , chromatography , sample (material) , matching (statistics) , computational biology , computer science , biology , microbiology and biotechnology , chemistry , proteomics , genetics , medicine , pathology , gene
Two‐dimensional electrophoresis (2‐DE) is one of the most commonly used techniques in proteomic investigations. However, due to the complex interplay of incidence including significant biological sample variations, lengthy steps involved in performing 2‐DE as well as exposure time with silver staining, it is sometimes difficult to differentiate authentic differences caused by drug treatment with those artifacts caused by sample variations, running conditions of 2‐DE as well as treatment time in silver staining etc. If we can compare pooled samples of control and treatment groups run in a single gel and stained together, we would be more comfortable with our findings. We propose here a low cost and highly effective method for locating differentially expressed proteins before and after drug treatment. This “two‐in‐one gel” technique might partially solve the problems mentioned above.