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Unique scanning capabilities of a new hybrid linear ion trap mass spectrometer (Q TRAP) used for high sensitivity proteomics applications
Author(s) -
Le Blanc J. C. Yves,
Hager James W.,
Ilisiu A. M. Patricia,
Hunter Christie,
Zhong Feng,
Chu Ivan
Publication year - 2003
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300415
Subject(s) - quadrupole ion trap , ion trap , mass spectrometry , top down proteomics , chemistry , ion , hybrid mass spectrometer , tandem mass spectrometry , trap (plumbing) , triple quadrupole mass spectrometer , analytical chemistry (journal) , selected reaction monitoring , proteomics , characterization (materials science) , mass spectrum , chromatography , materials science , nanotechnology , physics , organic chemistry , meteorology , biochemistry , gene
The unique scanning capabilities of a hybrid linear ion trap (Q TRAP) mass spectrometer are described with an emphasis on proteomics applications. The combination of the very selective triple quadrupole based tandem mass spectrometry (MS/MS) scans with the very sensitive ion trap product ion scans allows rapid identification of peptides at low concentrations derived from post‐translationally modified proteins on chromatographic time scales. The Q TRAP instrument also offers the opportunity to conduct a variety of ion processing steps prior to performing a mass scan. For example, the enhancement of the multiple‐charge ion contents of the ion trap can be performed resulting in a survey mass spectrum dominated by double‐ and triple‐charge peptides. This facilitates the identification of relevant biological species in both separated and unseparated peptide mixtures for further MS/MS experiments.