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Identification of new celiac disease autoantigens using proteomic analysis
Author(s) -
Stulík Jiří,
Hernychová Lenka,
Porkertová Stanislava,
Pozler Oldřich,
Tučková Ludmila,
Sánchez Daniel,
Bureš Jan
Publication year - 2003
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300370
Subject(s) - autoantibody , immunology , antibody , lamina propria , tissue transglutaminase , antigen , immune system , disease , immunoglobulin a , medicine , autoimmunity , human leukocyte antigen , coeliac disease , biology , immunoglobulin g , pathology , enzyme , biochemistry , epithelium
Abstract Celiac disease is an autoimmune disorder in which gluten peptides presented by specific HLA‐DQ2‐ and HLA‐DQ8‐positive antigen presenting cells elicit immune response in connective tissue of lamina propria. Immunoglobulin A (IgA) antiendomysial antibodies are specific for celiac disease and are used for screening, diagnosis and follow‐up of this disease with an almost 100% sensitivity and specificity. The major target antigen of IgA antiendomysial antibodies was identified as tissue transglutaminase; nevertheless, the existence of the additional unique celiac disease‐specific autoantigens is anticipated. In this study we have utilized a proteomic approach in order to search out new autoantigens recognized by serum antibodies of patients with active celiac disease. We report the detection of 11 proteins that were immunorecognized with various frequencies by sera of patients with celiac disease. Four autoantigens were identified by mass fingerprinting approach as actin, ATP synthase β chain and two charge variants of enolase α. While production of IgA antibodies against actin molecules were described earlier, the existence of autoantibodies to ATP synthase β chain and enolase α species in sera collected from patients with active celiac disease are described for the first time. These results are suggestive of the existence of additional celiac disease autoantigens with possible diagnostic utility.