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Shotgun collision‐induced dissociation of peptides using a time of flight mass analyzer
Author(s) -
Purvine Samuel,
Eppel* JasonThomas,
Yi Eugene C.,
Goodlett David R.
Publication year - 2003
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.200300362
Subject(s) - shotgun , collision induced dissociation , dissociation (chemistry) , mass spectrometry , chemistry , peptide , spectrum analyzer , shotgun proteomics , chromatography , collision , ion , tandem mass spectrometry , time of flight , analytical chemistry (journal) , computer science , proteomics , biochemistry , computer security , telecommunications , organic chemistry , gene
Parallel collision‐induced dissociation (CID) of peptides rather than serial, as is customary, results in loss of the obvious parent‐fragment ion lineage available from CID on a single ion. We report proof‐of‐principle results suggesting the feasibility of parallel peptide CID, referred to here as shotgun CID, for protein identification when using the measured mass accuracies available from a time of flight mass analyzer and currently available search routines such as SEQUEST. Additionally, we report that parent‐fragment ion lineage may be reconstructed from information encoded in the chromatographic single ion current traces of peptides.