Open AccessA role for two‐component signaling elements in the Arabidopsis growth recovery response to ethylene
Author(s) -
Binder Brad M.,
Kim Hyo Jung,
Mathews Dennis E.,
Hutchison Claire E.,
Kieber Joseph J.,
Schaller G. Eric
Publication year - 2018
Publication title -
plant direct
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.211
H-Index - 11
ISSN - 2475-4455
DOI - 10.1002/pld3.58
Subject(s) - response regulator , histidine kinase , ethylene , arabidopsis , cytokinin , microbiology and biotechnology , biology , signal transduction , histidine , mutant , two component regulatory system , kinase , biochemistry , gene , enzyme , auxin , catalysis
Previous studies indicate that the ability of Arabidopsis seedlings to recover normal growth following an ethylene treatment involves histidine kinase activity of the ethylene receptors. As histidine kinases can function as inputs for a two‐component signaling system, we examined loss‐of‐function mutants involving two‐component signaling elements. We find that mutants of phosphotransfer proteins and type‐B response regulators exhibit a defect in their ethylene growth recovery response similar to that found with the loss‐of‐function ethylene receptor mutant etr1‐7 . The ability of two‐component signaling elements to regulate the growth recovery response to ethylene functions independently from their well‐characterized role in cytokinin signaling, based on the analysis of cytokinin receptor mutants as well as following chemical inhibition of cytokinin biosynthesis. Histidine kinase activity of the receptor ETR 1 also facilitates growth recovery in the ethylene hypersensitive response, which is characterized by a transient decrease in growth rate when seedlings are treated continuously with a low dose of ethylene; however, this response was found to operate independently of the type‐B response regulators. These results indicate that histidine kinase activity of the ethylene receptor ETR 1 performs two independent functions: (a) regulating the growth recovery to ethylene through a two‐component signaling system involving phosphotransfer proteins and type‐B response regulators and (b) regulating the hypersensitive response to ethylene in a type‐B response regulator independent manner.