Open AccessTargeted mutagenesis of StISAC stabilizes the production of anthocyanins in potato cell culture
Author(s) -
D'Amelia Vincenzo,
Staiti Annalisa,
D'Orso Fabio,
Maisto Maria,
Piccolo Vincenzo,
Aversano Riccardo,
Carputo Domenico
Publication year - 2022
Publication title -
plant direct
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.211
H-Index - 11
ISSN - 2475-4455
DOI - 10.1002/pld3.433
Subject(s) - mutant , mutagenesis , repressor , crispr , anthocyanin , inducer , gene silencing , gene , cell culture , biology , biochemistry , biosynthesis , chemistry , genetics , food science , gene expression
To increase the production of decorated anthocyanins in potato cell cultures, we knocked out a novel potato gene, named Inducer Silencing of Anthocyanins in Cell culture ( StISAC ), using CRISPR‐Cas9 editing. Our results provided evidence that mutant cell lines doubled the accumulation level of anthocyanins biosynthesized. Moreover, the production of these important pigments was stabilized over time. Our study overcame important challenges in the efficient biotechnological production of these valuable pigments and reported the function of a novel anthocyanin biosynthesis repressor gene.