Open AccessPosttranscriptional regulation of cellulose synthase genes by small RNAs derived from cellulose synthase antisense transcripts
Author(s) -
Nething Daniel B.,
Sukul Abhijit,
MishlerElmore John W.,
Held Michael A.
Publication year - 2021
Publication title -
plant direct
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.211
H-Index - 11
ISSN - 2475-4455
DOI - 10.1002/pld3.347
Subject(s) - biology , gene , gene silencing , antisense rna , gene expression , hordeum vulgare , brachypodium distachyon , rna silencing , rna , regulation of gene expression , genetics , gene expression profiling , microbiology and biotechnology , rna interference , genome , ecology , poaceae
Transcriptional regulatory mechanisms governing plant cell wall biosynthesis are incomplete. Expression programs that activate wall biosynthesis are well understood, but mechanisms that control the attenuation of gene expression networks remain elusive. Previous work has shown that small RNAs (sRNAs) derived from the HvCESA 6 ( Hordeum vulgare , Hv ) antisense transcripts are naturally produced and are capable of regulating aspects of wall biosynthesis. Here, we further test the hypothesis that CESA ‐derived sRNAs generated from CESA antisense transcripts are involved in the regulation of cellulose and broader cell wall biosynthesis. Antisense transcripts were detected for some but not all members of the CESA gene family in both barley and Brachypodium distachyon . Phylogenetic analysis indicates that antisense transcripts are detected for most primary cell wall CESA genes, suggesting a possible role in the transition from primary to secondary cell wall biosynthesis. Focusing on one antisense transcript, HvCESA1 shows dynamic expression throughout development, is correlated with corresponding sRNAs over the same period and is anticorrelated with HvCESA1 mRNA expression. To assess the broader impacts of CESA ‐derived sRNAs on the regulation of cell wall biosynthesis, transcript profiling was performed on barley tissues overexpressing CESA ‐derived sRNAs. Together, the data support the hypothesis that CESA antisense transcripts function through an RNA‐induced silencing mechanism, to degrade cis transcripts, and may also trigger trans ‐acting silencing on related genes to alter the expression of cell wall gene networks.