Open AccessTwo members of the DUF 579 family are responsible for arabinogalactan methylation in Arabidopsis
Author(s) -
Temple Henry,
Mortimer Jenny C.,
Tryfona Theodora,
Yu Xiaolan,
LopezHernandez Federico,
Sorieul Mathias,
Anders Nadine,
Dupree Paul
Publication year - 2019
Publication title -
plant direct
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.211
H-Index - 11
ISSN - 2475-4455
DOI - 10.1002/pld3.117
Subject(s) - xylan , arabinogalactan , methylation , glucuronic acid , biochemistry , cell wall , chemistry , methyltransferase , arabidopsis , biosynthesis , enzyme , polysaccharide , gene , mutant
All members of the DUF579 family characterized so far have been described to affect the integrity of the hemicellulosic cell wall component xylan: GXMs are glucuronoxylan methyltransferases catalyzing 4‐O–methylation of glucuronic acid on xylan; IRX15 and IRX15L, although their enzymatic activity is unknown, are required for xylan biosynthesis and/or xylan deposition. Here we show that the DUF579 family members, AGM1 and AGM2, are required for 4‐O–methylation of glucuronic acid of a different plant cell wall component, the highly glycosylated arabinogalactan proteins (AGPs).