Bioreducible poly(amido amine) copolymers derived from histamine and agmatine for highly efficient gene delivery | Zendy

Sun Yanping | Zendy; Liu Hui | Zendy; Xing Haonan | Zendy; Lang Lang | Zendy; Cheng Lin | Zendy; Yang Tianzhi | Zendy; Yang Li | Zendy; Ding Pingtian | Zendy

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Bioreducible poly(amido amine) copolymers derived from histamine and agmatine for highly efficient gene delivery

Author(s) -

Sun Yanping,

Liu Hui,

Xing Haonan,

Lang Lang,

Cheng Lin,

Yang Tianzhi,

Yang Li,

Ding Pingtian

Publication year - 2019

Publication title -

polymer international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.592

H-Index - 105

eISSN - 1097-0126

pISSN - 0959-8103

DOI - 10.1002/pi.5728

Subject(s) - agmatine , gene delivery , transfection , endocytosis , chemistry , endosome , michael reaction , cytotoxicity , pinocytosis , cationic polymerization , amine gas treating , monomer , polymer , polymer chemistry , biophysics , combinatorial chemistry , biochemistry , amino acid , organic chemistry , gene , cell , biology , in vitro , arginine , catalysis

Histamine (HIS) can facilitate the endosomal escape of polyplexes via the ‘proton sponge effect’ because of its imidazole groups. Agmatine (AGM) can improve the transmembrane process of polyplexes as a result of its guanidinium groups. Therefore, HIS and AGM were used as amino monomers to react with cystamine bisacrylamide (CBA) through Michael addition. The synthesized peptide‐mimicking poly(CBA‐HIS/AGM)s showed high transfection efficiency and low cytotoxicity, indicating their great potential as gene carriers. The results also demonstrated that the effects of HIS and AGM on the properties of poly(CBA‐HIS/AGM)s were different: HIS could increase their buffering capacities and bioreducibility, but AGM could facilitate their plasmid DNA packaging and condensing abilities. In addition, the results of transfection mechanism studies indicated that poly(CBA‐HIS/AGM) polyplexes entered into cells mainly via clathrin‐dependent endocytosis and they could efficiently escape the endosome, indicating endosomal escape was not the limiting step for gene delivery based on these polymers. © 2018 Society of Chemical Industry

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