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An in vitro focus on doxorubicin hydrochloride delivery of novel pH‐responsive poly(2‐succinyloxyethylmethacrylate) and poly[( N ‐4‐vinylbenzyl), N , N ‐diethylamine] diblock copolymers
Author(s) -
Ghamkhari Aliyeh,
Massoumi Bakhshali,
Agbolaghi Samira
Publication year - 2018
Publication title -
polymer international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.592
H-Index - 105
eISSN - 1097-0126
pISSN - 0959-8103
DOI - 10.1002/pi.5504
Subject(s) - copolymer , zeta potential , polymer chemistry , polymerization , doxorubicin hydrochloride , diethylamine , cationic polymerization , raft , chemistry , dynamic light scattering , nuclear chemistry , mtt assay , chain transfer , polymer , materials science , in vitro , doxorubicin , organic chemistry , radical polymerization , nanoparticle , nanotechnology , biochemistry , medicine , surgery , chemotherapy
Novel pH‐responsive poly(2‐succinyloxyethylmethacrylate)‐ b ‐poly[( N ‐4‐vinylbenzyl), N , N ‐diethylamine] [poly(SEMA‐ b ‐VEA)] diblock copolymers were synthesized via reversible addition fragmentation transfer (RAFT) polymerization to investigate their self‐assembly micellar behavior. The self‐assembly behaviors of synthesized diblock copolymers with distinct molecular weights (labeled (1) to) were confirmed by 1 H NMR spectroscopy, TEM and dynamic light scattering measurements. Doxorubicin hydrochloride (DOX) loading capacity was evaluated, and the in vitro cytotoxicity effect of DOX‐loaded diblock copolymer was also studied by assessing the survival rate of the breast cancer cell line MCF‐7 with 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay. The results exhibited remarkable controlled release in the MTT assay. The DOX encapsulation efficiency was calculated to be 96.4%. The size and zeta potential of DOX‐loaded poly(SEMA‐ b ‐VEA) diblock copolymers were 204 nm and +5.7 mV at a pH of 7.4. DOX release values after 440 h at pH 7.4, 5.4 and 4 were 22.15%, 31.43% and 47.06%, respectively. The released values of DOX‐loaded poly(SEMA‐ b ‐VEA) and at pH 7.4 were 22.15%, 20.5% and 17.5%, respectively. Cell survival ratios were 18.9%, 23.16% and 16.92% after 72 h. Poly(SEMA‐ b ‐VEA) copolymers can be considered in nanomedicine applications due to their excellent pH‐responsive micellar behavior. © 2017 Society of Chemical Industry