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Effecters on human placental alkaline phosphatase activity
Author(s) -
Roig M. G.,
Ghaïs N. I.,
Burguillo F. J.,
Cachaza J. M.,
Kennedy J. F.
Publication year - 1993
Publication title -
polymer international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.592
H-Index - 105
eISSN - 1097-0126
pISSN - 0959-8103
DOI - 10.1002/pi.4990310205
Subject(s) - alkaline phosphatase , substrate (aquarium) , chemistry , enzyme , placental alkaline phosphatase , albumin , ionic strength , phosphatase , biochemistry , human serum albumin , serum albumin , human albumin , catalysis , sodium , enzyme assay , biology , organic chemistry , ecology , aqueous solution
A kinetic study has been carried out on the effects of enzyme concentration, protein (human serum albumin) concentration and ionic strength (inorganic and organic sodium salts) on the activity of human placental alkaline phosphatase in solution. The activity is depressed by serum albumin, and the corresponding complex of the protein with the substrate, decreasing its concentration available for catalysis, is responsible for an effect similar to competitive inhibition of the alkaline phosphatase activity. Activating and inhibiting effects were found for inorganic anions, respectively.